Dhabaleswar Patra, Ph.D.

Senior Scientist
Structural Biology & Bioinformatics
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Research Focus

Cardiovascular disease, Signal transduction, Structural Biology (Crystallography & Cryo-EM)

Research Interest

Our lab research interests are in understanding molecular mechanisms of cardiovascular disease. To understand this, we study proteins involved in cell adhesions during macrophages recruitment and plaque formation, signaling proteins involved in heart disease progression and proteins involved in reverse atherosclerosis. We use a combination of techniques to accomplish our goals including Cryo-Electron microscopy, X-ray crystallography and various functional assays. Our ultimate goal is to use the information from the structural and functional characterization as a starting point for inhibitor design against CVDs and to develop bio-marker for predicting  heart attack at an early stages.

Credentials

  1. Senior Scientist (2023-onwards)
  2. Post-doctoral Research Fellow (2018-2022) Purdue University, West Lafayette, Indiana, USA
  3. Post-doctoral Research Fellow (2015-2017) University of Michigan Ann Arbor, Michigan, USA
  4. Ph.D (2014) Indian Institute of Science, Bangalore, India 

 

Patents & Publications

Publications:

  1. Chen Q, Plasencia M, Zhuang Li, Mukherjee S, Patra D, Chen CL, Klose T, Yao X, Chang L, Kossiakof AA, Chang L, Andrews PC, Tesmer JJG. Structure of a Rhodopsin in complex with G-protein-coupled receptor kinase 1. Nature. 2021 Jul 14. doi: 10.1038/s41586-021-03721-x.
  2. Plescia CB, David EA, Patra D, Sengupta R, Amiar S, Su Y, and Stahelin RV. SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles. JBC 2020 Nov 19.  doi: 10.1074/jbc.RA120.016148
  3. Manthei KA, Patra D, Wilson CJ, Fawaz MV, Piersimoni L, Shenkar J, Yuan W, Andrews PC, Engen JR, Schwendeman A, Ohi MD, Tesmer JJG. Structural analysis of lecithin:cholesterol acyltransferase bound to high density lipoprotein particles.  Commun Biol 3, 28 (2020) doi:10.1038/s42003-019-0749-z.  
  4. Beyett TS, Fraley AE, Labudde E, Patra D, Coleman RC, Eguchi A, Glukhova A, Chen Q, Williams RM, Koch WJ, Sherman DH, Tesmer JJG. Perturbation of the interactions of calmodulin with GRK5 using a natural product chemical probe. PNAS. 2019 Aug 6; 116(32):15895-15900 
  5. Seegar TCM, Killingsworth LB, Saha N, Meyer PA, Patra D, Zimmerman B, Janes PW, Rubinstein E, Nikolov DB, Skiniotis G, Kruse AC, Blacklow SC. Structural Basis for Regulated Proteolysis by the α-Secretase ADAM10. Cell. 2017 Dec 14;171(7):1638-1648.
  6. Komolov KE, Du Y, Duc NM, Betz RM, Rodrigues JPGLM, Leib RD, Patra D, Skiniotis G, Adams CM, Dror RO, Chung KY, Kobilka BK, Benovic JL. Structural and Functional Analysis of a β2-Adrenergic Receptor Complex with GRK5. Cell. 2017 Apr 20;169(3):407-421
  7. Das M, Du Y, Ribeiro O, Hariharan P, Mortensen JS, Patra D, Skiniotis G, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PS. Conformationally preorganized diastereomeric norbornane-based maltosides (NBMs) for membrane protein study: Implications of detergent kink for micellar properties. J Am Chem Soc. 2017 Mar 1;139(8):3072-3081. 
  8. Dasgupta N, Ranjan S, Patra D, Srivastava P, Kumar A, Ramalingam C. Bovine serum albumin interacts with silver nanoparticles with a "side-on" or "end on" conformation. Chem Biol Interact. 2016 253:100-11. 
  9. Patra D, Mishra P, Vijayan M, Surolia A. Negative cooperativity and high affinity in chitooligosaccharide binding by a Mycobacterium smegmatis protein containing LysM and lectin domains. Biochemistry, 2015 55: 49-61. 
  10. Patra D, Mishra P, Surolia A, Vijayan M. Structure, interactions and evolutionary implications of a domain-swapped lectin dimer from Mycobacterium smegmatis. Glycobiology, 2014 24: 956-965.
  11. Patra D, Sharma A, Chandran D, Vijayan M. Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose- binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2011 67:596-599. 
  12. Patra D, Srikalaivani R, Misra A, Singh DD, Selvaraj M, Vijayan M. Cloning, expression, purification, crystallization and preliminary X-ray studies of a secreted lectin (Rv1419) from Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2010 66:1662-1665.