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Drug Development Diagnostics & Biotechnology Division

Dr. Samir Kumar Dutta

Principal Scientist  
Ph.D. (Pharm), Jadavpur University1986
Contact - samirkdutta@iicb.res.in / somir_india@yahoo.com

Current Research Interest

The basic concept is to convert knowledge-based science achieved in this Lab to the technology of tomorrow. The key to such concept relies on: -

  • Identification and characterization of proteinaceous amylase and/ or protease inhibitors from non-host plant sources for the polyphagous-pest, Helicoverpa armigera. Two protease inhibitor genes and an amylase/ trypsin inhibitor gene have been cloned recently (Sequences submitted to GenBank).
  • Artificial feeding with these inhibitors proved detrimental to armigera (Collaboration).
  • Complete peas plant regenerated as a key to incorporation and expression of the above genes in peas/ chickpea through Agrobacterium.
  • Potential of these inhibitors as tumoricidal, MMP-inhibitor, t-PAI etc. is also under consideration.
  • Protein Engineering (Synthesis of designer proteins) is another aim of this Lab.
  • Biotransformation of Plant Secondary metabolites.
  • Regeneration of complete winged bean plants through various stages of tissue culture up to  the pod formation stage in field. Initiated for the RNAi mediated suppression of the winged bean protease inhibitor genes mentioned above for understanding the hitherto unknown physiological functions of these inhibitors
  • Development of anticancer formulation and synthetic analogues is another aim of this Lab.

Names of the group members including regular staff with designation and research fellows:
  Fellow/RA/Proj.Asstt./Others  
 
  1. Dr. Saswati Sen, Principal Investigator,DST WOS-A
  2. Ms. Sayanika Banerjee, Project assistant level-II
  3. Mr. Monisankar Ghosh, JRF (UGC)
  4. Ms. Suchandrima Saha, JRF (CSIR)
 

List of important Publications:

  1. Dattagupta, J. K., Chakraborti C., Podder A., Dutta S. K. and Singh, M. (1990) Crystallization and preliminary X-ray studies of Psophocarpin B1, a chymotrypsin inhibitor from winged bean seeds J. Mol. Biol. 216, 229-231.
  2. Ray, M., Halder, J., Dutta, S. K. and Ray, S. (1991) Inhibition of Respiration of Tumor Cells by Methyl Glyoxal; Protection   by Lactaldehyde. Int. J. Cancer, 47, 603-619.
  3. Maity, S. K., Dutta, S. K., Banerjee, A. K., Achari, B. and Singh, M. (1994) Design and synthesis of mannose analogues as inhibitors of mannosidase. Tetrahedron, 50, 6965-6974.
  4. Dattagupta, J. K. Podder, A., Chakraborti, C., Sen, U., Dutta, S. K. and Singh, M. (1996)          Acta. Crystallographica D 52, 521-528.
  5. Hirabayashi, J., Dutta, S. K. and Kasai, K. (1998) Novel galactose-binding   proteins in Annelida; J. Biol. Chem. 273 (23), p. 14450-14460.
  6. Dattagupta, J. K., Podder, A., Chakrabarti C., Sen, U., Mukhopadhyay, D., Dutta, S. K. and Singh, M. (1999) Refined crystal structure (2.3 Å) of a double-headed winged bean alpha-chymotrypsin inhibitor and location of its second reactive site. Proteins:Structure Function,Genetics.  35 (3), 321-331.
  7. Datta, K., Usha, R., Dutta, S. K. and Singh, M. (2001) A comparative study of the winged bean protease inhibitors and their interaction with proteases. Plant Physiol. and Biochem. 39, 949-959.   
  8. Roy, S. and Dutta S. K. (2009) Purification of a chymotrypsin-trypsin inhibitor from winged bean seeds using single-step Immuno-affinity column. Am. J. Biochem and Biotech. 5, No.3, p.142-146.
  9. Roy, S. and Dutta S. K. (2009) Genomic and cDNA cloning, expression, purification and characterization of a chymotrypsin-trypsin inhibitor from winged bean seeds. Biosci. Biotech. Biochem. 73, No. 12, 2671-2676. 
  10. Roy, S., Mandal, C. N. and Dutta, S. K. (2011) Site-directed Mutagenesis to Identify Key Residues in Structure-Function Relationship of Winged bean Chymotrypsin-Trypsin Inhibitor and 3-D Structure Prediction. Protein and Peptide Letters, Volume 18, Number 5, May, 471-479.
  11. Mukherjee, S., Chaki, S., Das, S., Sen, S. Dutta, S. K. Dastidar, S.G. (2011) Distinct Synergestic action of Piperacillin and Methyl glyoxal against Psuudomonas aeruginosa Ind. J. Expt. Biol. Vol. 49, p. 547-551.
  12. Sen, S., Dutta, S. K. and Dastidar, S. G.  (2011) Development of a Highly Potent Therapeutic Regimen for Chronic Myeloid Leukemia using the Extract of Eleusine coracana Seeds. Ind. J. Biomed. Pharm. Sci. 5, No. 1, 7-11.
  13. Sen, S. and Dutta, S. K. (2011) Cloning, expression and characterization of biotic stress inducible Ragi bifunctional inhibitor (RBI) gene from Eleusine coracana Gaertn. J. Plant Biochem. Biotechnol. [ DOI 10. 1007/s 13562-011-0082-1]

 
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