Cancer Biology & Inflammatory Disorder Division

Dr. Chitra Mandal

Ph.D, FTWAS, F.N.A.,F.A.Sc, F.N.A.Sc, FAMS, J.C. Bose National Fellow

Contact: chitra_mandal@yahoo.com / cmandal@iicb.res.in


Outstanding Scientist & Acting Director

 

Current Research Interest

Prof. Chitra Mandal’s group is actively working in the field of different aspect of disease biology mainly in cancer, leishmanial and bacterial infection as described below:

Glycobiology in pathogenic infection and cancer

  • Glycobiological approach to understand the altered host innate immune response in leishmanial (L. donovani) and bacterial (P. aeruginosa) infection
  • Proteomic and glycoproteomic approach to identify and validate the disease-associated proteins/glycoproteins and their importance in disease pathogenesis in both leishmanial and bacterial infection
  • Proteomic and glycoproteomic study to identify and validate the disease specific proteins and their importance in cancer development  
  • Define cancer cells by glycoenzymology (glycosylation regulating enzymes) and establish the early response disease specific enzymes as diagnostic markers

Cancer cell signalling, target identification and therapeutics

  •  Understand the tumor progression and regression in terms of signal cross-talking and development of novel anticancer chemotherapeutics

Perspective of CSCs in cancer and development of futuristic therapy against CSCs 

  • Understanding of cancer stem cells (CSCs) in glioblastoma multiforme and acute lymphoblastic leukemia in terms of immunophenotype, molecular and developmental aspect
  • Designing of the targeted therapeutic protocol and drug delivery systems to overcome CSCs dependent drug resistance and disease relapse

T-cell immunology in cancer

  • Understand the T regulatory (Treg) cell mediated immune response in B-cell acute lymphoblastic leukemia (Human and mouse model)

 

Names of the group members including regular staff with designation and research fellows

Staff Member`s

Mr. Asish Mullick Extn.No.901
Sr. Technical Officer (2)

Project Fellows

Ms. Rita Maity
Project Assistant Lavel-II

Research Fellows

Dr. Chandan Mandal
Research Associate
Dr. Manjusha Chakrobarty
Research Associate
Mr. Biswajit Khatua
Extended Senior Research Fellow
Mr. Suman Kr. Samanta
CSIR - Senior Research Fellow
Mr. Saptarshi Roy
CSIR - Senior Research Fellow
Ms. Arup Kr. Bag 
ICMR - Senior Research Fellow
Ms. Devawati Dutta
CSIR - Senior Research Fellow
Mr. Samarpan Maiti
UGC-Junior Research Fellow
MS Joyshree Karmakar
JRF

MS Shalini Nath

JRF

MS Susmita Mondal

JRF
Mr. Kaustuv Mukherjee
JRF

Lab Assistant

Mr. Karu Ravi Das

List of important Research Articles

  1. Biswajit Khatua, Jeremy Van Vleetb, Biswa Pronab Choudhuryb, Chitra Mandal (2014) Sialylation of OprD protein: A mechanistic basis of antibiotic uptake in Pseudomonas aeruginosa. Molecular & Cellular Proteomics Accepted in March 18
  2. Sayantani Sarkar, Chandan Mandal, Rajender Sangwan and Chitra Mandal  (2014) Chk1/Chk2 couples with G2/M cell cycle arrest and perturbed canonical Wnt/β-catenin pathway to elicit apoptosis in pancreatic adenocarcinoma’  Endrocine Related Cancer, 21, 113-125 
  3. Chandan Mandal, Sayantani Sarkar, Uttara Chatterjee, Reinhard Schwartz-Albiez, Chitra Mandal (2014) Disialoganglioside GD3-synthase over expression inhibits survival and angiogenesis of pancreatic cancer cells through cell cycle arrest at S-phase and disruption of integrin-β1-mediated anchorage The Int J Biochem Cell Biol, May 16th pii: S1357-2725(14)00173-3,
  4. Ranjita Das, Kaushik Bhattacharya, Suman K Samanta, Bikas C Pal and Chitra Mandal (2014) Improved chemosensitivity in cervical cancer to cisplatin: synergistic activity of mahanine through STAT3 inhibitionCancer Letters May 14. pii: S0304-3835(14)00260-2. doi: 10.1016/j.canlet.2014.05.005
  5. Ranjita Das, Kaushik Bhattacharya, Sayantani Sarkar, Suman K Samanta, Bikas C Pal and Chitra Mandal (2014) Mahanine synergistically enhances cytotoxicity of 5-fluorouracil through ROS-mediated activation of PTEN and p53/p73 in colon carcinoma Apoptosis 19:149-164 DOI: 10.1007/s10495-013-0907-6 
  6. Bhattacharya, Kaushik; Chandra, Sarmila; Mandal, Chitra (2014) Critical Stoichiometric ratio of CD4+CD25+FoxP3+ Treg and CD4+CD25- Tresp persuades immunosuppression in patient with B-cell Acute Lymphoblastic Leukemia Immunology May;142(1):124-39. doi: 10.1111/imm.12237
  7. R.K Mehta, S Verma, S Mohanty,  P. Jena, B. Khatua, R. Jena, S. Sethy, Chitra Mandal, K.H. Roehm, and A. Sonawane (2014) Mutations in subunit interface and B-cell epitopes improve antileukemic activities of Escherichia coli asparaginase-II: Evaluation of immunogenicity in mice. J. Biol. Chem 289 (6), 35555-70
  8. Saptarshi Roy, G. Aditya Kumar, Md. Jafurulla, Chitra Mandal, Amitabha Chattopadhyay (2014) Integrity of the Actin Cytoskeleton of Host Macrophages is Essential for Leishmania donovani Infection. Biochim Biophys Acta. 2014 Apr 26;1838(8):2011-2018. doi: 10.1016/j.bbamem.2014.04.017
  9. C. D. P. Tripathi, R. Gupta, P. K. Kushawaha, Chitra Mandal, S. Misra Bhatracharya & A. Dube (2014) Efficacy of Withania somnifera chemotypes NMITLI–101R, 118R and Withaferin A against experimental visceral leishmaniasis Parasite Immunology 1-13
  10. Das M, Bhattacharya K, Dittrick SA, Mandal Chitra, Balla VK, Sampath Kumar TS, Bandyopadhyay A, Manna I (2014). In situ synthesized TiB-TiN reinforced Ti6Al4V alloy composite coatings: Microstructure, tribological and in-vitro biocompatibility. J Mech Behav Biomed Mater. Jan 29:259-271. doi: 10.1016/j.jmbbm.2013.09.006.  
  11. Sarita Roy, Kaushik Bhattacharya, Chitra Mandaland Anjan K. Dasgupta (2013) Cellular response to chirality and amplified chirality. Journal of Materials Chemistry B. 1:6634-43 DOI: 10.1039/C3TB21322F Impact factor 6.01
  12. Suman K. Samanta, Devawati Dutta, Sarita Roy, Kaushik Bhattacharya, Sayantani Sarkar, Bikas C. Pal, Chhabinath Mandal, Anjan K. Dasgupta and Chitra Mandal(2013) Mahanine, a DNA minor grove binding agent exerts cellular cytotoxicity with involvement of C-7-OH and -NH functional groups.  Journal of Medicinal Chemistry, 56(14):5709-21. doi: 10.1021/jm400290q.
  13. S. Sarkar, D. Dutta, S.K Samanta, K. Bhattacharya, B.C Pal, J. Li, K. Datta, CN Mandal, and Chitra Mandal (2013) Redox sensitive inhibition of Hsp90 coupled with disruption of super-chaperone complex attenuate pancreatic adenocarcinoma in vitro and in vivo Int. J. Cancer 132:695-706. doi: 10.1002/ijc.27687. (One figure has been selected for cover page of the journal)
  14. Chandan Mandal and Chitra Mandal (2013) Identification and analysis of O-acetylated glycoproteins, A chapter in “Methods in Molecular Biology” Chapter 6, Series Editor Dr. John Walker, Human Press, Springer publishing group, USA 981:57-93.
  15. Biswajit Khatua, Saptarshi Roy and Chitra Mandal (2013) Sialic acids siglec interaction: A unique strategy to circumvent innate immune response by pathogens. An invited review  Indian Journal of Medical Research 138(5):648-62
  16. Das M, Bhattacharya K, Dittrick SA, Mandal C, Balla VK, Sampath Kumar TS, Bandyopadhyay A, Manna I. In situ synthesized TiB-TiN reinforced Ti6Al4V alloy composite coatings: Microstructure, tribological and in-vitro biocompatibility.J MechBehav Biomed Mater. 2013 Sep 18;29C:259-271. doi: 10.1016/j.jmbbm.2013.09.006. [Epub ahead of print]
  17. Das R, Bhattacharya K, Sarkar S, Samanta SK, Pal BC, Mandal C. Mahanine synergistically enhances cytotoxicity of 5-fluorouracil through ROS-mediated activation of PTEN and p53/p73 in colon carcinoma. Apoptosis. 2013 Sep 20. [Epub ahead of print]
  18. Samanta SK, Dutta D, Roy S, Bhattacharya K, Sarkar S, Dasgupta AK, Pal BC, Mandal C, Mandal C. Mahanine, A DNA Minor Groove Binding Agent Exerts Cellular Cytotoxicity with Involvement of C-7-OH and -NH Functional Groups. J Med Chem. 2013 Jul 5. [Epub ahead of print]
  19. Mandal C, Mandal C. Identification and analysis of o-acetylated sialoglycoproteins. Methods Mol Biol. 2013;981:57-93.
  20. Samanta S, Ghoshal A, Bhattacharya K, Saha B, Walden P, Mandal C. Sialoglycosylation of RBC in visceral leishmaniasis leads to enhanced oxidative stress, calpain-induced fragmentation of spectrin and hemolysis. PLoS One. 2012;7(7):e42361.
  21. Sarkar S, Dutta D, Samanta SK, Bhattacharya K, Pal BC, Li J, Datta K, Mandal C, Mandal C. Oxidative inhibition of Hsp90 disrupts the super-chaperone complex and attenuates pancreatic adenocarcinoma in vitro and in vivo. Int J Cancer. 2013 Feb 1;132(3):695-706.
  22. Mondal S, Roy S, Maity R, Mallick A, Sangwan R, Misra-Bhattacharya S, Mandal C. Withanolide D, carrying the baton of Indian rasayana herb as a lead candidate of antileukemic agent in modern medicine. Adv Exp Med Biol. 2012;749:295-312. Review.
  23. S Mondal, K. Bhattacharya, A. Mallick, R. Sangwan and Chitra Mandal (2012) Bak compensated for Bax in p53-null Cells to Release Cytochrome c for the Initiation of Mitochondrial Signaling during Withanolide D-induced Apoptosis.PLoS ONE 7(3): e34277. Epub 2012 Mar 29
  24. B. Khatua, K. Bhattacharya and Chitra Mandal (2012) α2,3 linked Sialic acids acquired by Pseudomonas aeruginosa facilitate their survival by impeding neutrophil extracellular trap through siglec-9.  Journal of Leucocyte Biology, 91, 641-55. Epub Jan 11
  25. Chandan Mandal,  Chhabinath Mandal, S. Chandra,  R. Schauer and Chitra Mandal (2012) Regulation of O-acetylation of sialic acids by sialate-O-acetyltransferase and sialate-O-acetylesterase activities in childhood acute lymphoblastic leukemia Glycobiology 22:70-83
  26. S. Kushwaha, S. Roy, R. Maity, A. Mallick, VK. Sonia, PK. Singha, ND. Chaurasiya, R. S. Sangwan, S. Misra-Bhattacharya and Chitra Mandal (2012) Chemotypical variations in Withania somnifera lead to differentially modulated immune response in BALB/c mice. Vaccine, 30, 1077-1087
  27. Chandan Mandal and Chitra Mandal (2012) Identification and analysis of O-acetylated glycoproteins, A chapter in “Methods in Molecular Biology” Chapter 6, Series Editor Dr. John Walker, Human Press, Springer publishing group, USA (in press)
  28. Chowdhury S, Mandal C, Sarkar S, Bag AK, Vlasak R, Chandra S, Mandal Chitra (2012) Mobilization of lymphoblasts from bone marrow to peripheral blood in childhood acute lymphoblastic leukaemia: Role of 9-O-acetylated sialoglycoproteins. Leukemia Res 36, 146-55
  29. S. Samanta, D Dutta, A Ghoshal, S Mukhopadhyay, B Saha, S Sundar, S Jarmalavicius, M Forgber, CN Mandal, P Walden and Chitra Mandal (2011) Glycosylation of erythrocyte spectrin and its modification in visceral leishmaniasis PLOS one, Accepted
  30. Chandan Mandal,  Chhabinath Mandal, S. Chandra,  R. Schauer and Chitra Mandal (2011) Regulation of O-acetylation of sialic acids by sialate-O-acetyltransferase and sialate-O-acetylesterase activities in childhood acute lymphoblastic leukemia Glycobiology, in press
  31. Mandal Chitra, Roychoudhury S, Roy S. (2011) Cancer research: India meets the West. Cell Death Differ. Jun 17. doi: 10.1038/cdd.2011.85
  32. S. Mondal, S. Bandyopadhyay, M. K Ghosh, S. Mukhopadhyay, S. Roy and Chitra Mandal (2011). Natural products: Promising resources for cancer drug discovery. Anti Cancer Agents in Medicinal Chemistry, (Formerly Current Medicinal Chemistry - Anti-Cancer Agents) Jun 27. [Epub ahead of print], PMID: 21707502 [PubMed - as supplied by publisher
  33. Chitra Mandal, R. Schwartz-Albiez and R. Vlasak (2011) Functions and biosynthesis of O-acetylated sialic acids. A Review "Topics in Current Chemistry" Volume: SialoGlyco Chemistry and Biology, Volume Editors: Rita Gerardy-Schahn, Philippe Delannoy, Mark von Itzstein (accepted) 
  34. S. Mondal, S. Roy, R. Maity, A. Mallick, R. Sangwan, S. Misra-Bhattacharya and Chitra Mandal (2011) Withanolide-D, carrying the baton of Indian Rasayana herb as a lead candidate of anti-leukemic agent in modern medicine. Adv Expl Med and Biol, Kluwer Academic Publishers.
  35. A. Ghoshal and Chitra Mandal (2011) A perspective on the emergence of sialic acids as potent determinants affecting Leishmania biology. Molecular Biology International (accepted)
  36. K. Mukherjee, S. Chowdhury, S. Mondal, Chandan Mandal, S. Chandra and Chitra Mandal (2011) 9-O-Acetyl GD3 in lymphoid and erythroid cells. Adv Expl Med and Biol 705:317-334, Kluwer Academic/Plenum Publishers, New York, USA 
  37. Angana Ghoshal, Gerrit J. Gerwig, Johannis P. Kamerling, and Chitra Mandal (2010) Sialic acids in different Leishmania spp., its correlation with nitric oxide resistance and host responses.  Glycobiology  (accepted)
  38. Kaushik Bhattacharya, Suman K. Samanta, Rakshamani Tripathi, Asish Mallick, Sarmila Chandra, Bikas C. Pal, Chandrima Shaha and Chitra Mandal (2010) Apoptotic effects of mahanine on human leukemic cells are mediated through cross talking between Apo-1/Fas signaling with Bid protein and via mitochondrial pathways. Biochemical Pharmacology 79: 361-72.
  39. Chandan mandal, Cristina Tringali, Susmita Mondal, Leugi Anastasia, Sarmila Chandra, Venerando Burno and Chitra Mandal (2010) Down-regulation of membrane-bound Neu3 is negatively correlated with disease progression and associated with apoptosis suppression of lymphoblasts in childhood acute lymphoblastic leukemia. International J Cancer 126:337-349
  40. Jain R, Ghoshal A, Mandal Chitra, Shaha C. (2009) Leishmania cell surface prohibitin: role in host-parasite interaction. Cell Microbiol. Nov 2. [Epub ahead of print]  PMID: 19888987
  41. Khatua B, Ghoshal A, Bhattacharya K, Mandal C, Saha B, Crocker PR, Mandal Chitra. (2009). Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition.  FEBS Lett. Nov 27. [Epub ahead of print] PMID: 19945458
  42. Susmita Mondal, Chandan Mandal, Sarmila Chandra and Chitra Mandal (2009) Elevated mRNA level of hST6Gal I and hST3Gal V positively correlates with the high risk of pediatric acute leukemia. Leukemia Research     Aug 24. [Epub ahead of print] PMID: 19709745
  43. Rajdeep Chowdhury, Suchandra Chowdhury, Paromita Roychoudhury, Chitra Mandal and Keya Chaudhuri (2009) Arsenic induced apoptosis in malignant melanoma cells is enhanced by menadione through ROS generation, p38 signaling and p53 activation. Apoptosis 14, 108-123
  44. Aparajita Pal, Iman Bhattacharya, Kaushik Bhattacharyya, Chitra Mandal, Manju Ray (2009) Methylglyoxal induced activation of murine peritoneal macrophages and surface markers of T lymphocytes in Sarcoma-180 bearing mice: Involvement of MAP kinase, NF kb signal transduction pathway. Molecular Immunology 46, 2039-44
  45. Angana Ghoshal, Sumi Mukhopadhyay, Rodion Demine, Michael Forgber, Saulius Jarmalavicius, Bibhuti Saha, Shyam Sundar, Peter Walden, Chhabinath Mandal, and Chitra Mandal (2009) Detection and characterization of a sialoglycosylated bacterial ABC-type phosphate transporter protein from patients with visceral leishmaniasis. Glyconjugate Journal 26, 675-89.
  46. Waliza Ansar, Sumi Mukhopadhyay, Shyamasree Basu, SK. Hasan Habib, Bibhuti Saha, Asish Kumar Sen, Chhabinath Mandal, and Chitra Mandal (2009) Disease-associated glycosylated moleclar variants of human C-reactive protein activate complement-mediated hemolysis of erythrocytes in tuberculosis and Indian Visceral leishmaniasis. Glycoconjugate J 26, 1151
  47. Chandan Mandal, G. Vinayaga Srinivasan, Suchandra Chowdhury, Sarmila Chandra, Chhabinath Mandal, Roland Schauer, and Chitra Mandal (2009) High Level of Sialate-O-acetyltransferase Activity in Lymphoblasts of Childhood Acute Lymphoblastic Leukaemia (ALL): Enzyme Characterization and Correlation with Disease Status. Glycoconjugate Journal 26, 57-73
  48. Waliza Ansar, SK. Hasan Habib, Samir Roy, Chhabinath Mandal and Chitra Mandal (2009). Unraveling the C-reactive protein complement-cascade in destruction of red blood cells: Potential Pathological Implications In Plasmodium falciparum Malaria. Cellular physiol biochem 23, 175-190
  49. Kankana Mukherjee, Anil  K  Chava, Suman  Bandyopadhyay, Asish  Mallick, MSc; Sarmila Chandra, Chitra mandal (2009). Co-expression of 9-O-acetylated sialoglycoproteins and their binding proteins on lymphoblasts of childhood acute lymphoblastic leukaemia: an anti-apoptotic role. Biol Chemistry 2009 390, 325-335
  50. Angana Ghoshal, Sumi Mukhopadhyay, Bibhuti saha, and Chitra Mandal (2009) 9-O-acetylated sialoglycoproteins: Important immunomodulators in Indian visceral leishmaniasis, Clinical and Vaccine Immunology 18, 889-898
  51. Sambuddha Banerjee, Susmita Mondal, Soma Sen, Saurabh Das, David L. Hughes, Corrado Rizzoli, Cédric Desplanches, Chitra Mandal and Samiran Mitra (2009) Four New Dinuclear Cu(II) Hydrazone Complexes Using Various Organic Spacers: Syntheses, Crystal Structures, DNA Binding and Cleavage Studies and Selective Cell Inhibitory Effect Towards Leukemic and Normal Lymphocytes.  Dalton Trans, 4:6849-60.
  52. Chandan Mandal, Avijit Dutta, Asish Mallick, Sarmila Chandra, Laxminarain Misra, Rajender  Sangwan, and Chitra Mandal (2008) Withaferin A induces apoptosis by activating p38 mitogen-activated protein kinase signaling cascade in leukemic cells of lymphoid and myeloid origin in a transcription-dependent manner through mitochondrial death cascade.  Apoptosis. 13, 1450-1464
  53. Kankana Mukherjee, Anil Kumar Chava, Chandan Mandal, Sailendra Nath Dey, Bernhard Kniep, Sarmila Chandra, Chitra Mandal (2008) O-acetylation of GD3 prevents its apoptotic effect and promotes survival of lymphoblasts in childhood acute lymphoblastic leukaemia. J. Cell biochem, 2008 105, 724-34
  54. Suchandra Chowdhury, Suman Bandyopadhyay, Chandan Mandal, and Sharmila Chandra, and Chitra Mandal  (2008) Flow-cytometric monitoring of disease-associated expression of 9-0 acetylated sialoglycoproteins in combination with known CD antigens, as an index for MRD in children with acute lymphoblastic leukaemia: a two-year longitudinal fllow-up study BMC Cancer, 8, 40
  55. Angana Ghoshal, Sumi Mukhopadhyay nee Bandyopadhyay and Chitra Mandal (2008) sialoglycotherapeutics in protozoal diseases Mini-Reviews in Medicinal Chemistry, Bentham Science Publishers, 8, 358-369
  56. Avijit Dutta, Debjani Sarkar, Ameenah Gurib-Fakim, Chitra Mandal* and Mitali Chatterjee (2008) In vitro and in vivo activity of Aloe vera leaf exudate in experimental visceral leishmaniasis. Parasitol Res 102, 1235-42
  57. Sumi Mukhopadhyay nee Bandyopadhyay and Chitra Mandal (2008) Targeting glycoproteins or glycolipids and their metabolic pathways for anti-parasite therapy, Review, Advances in Experimental Medicine and Biology   625: 87-102.
 
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Updated on 29th August 2014


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